Alteration in the activity of oxidative enzymes in the tissues of male Wistar albino rats exposed to cadmium.

OBJECTIVE The objective of the present study was to investigate the effect of cadmium (Cd) on the activities of some oxidative enzymes [viz Aldehyde oxidase, AO (E.C. 1.2.3.1); Xanthine oxidase, XO (E.C. 1.2.3.2); Sulphite oxidase, SO (E.C.1.8.3.1.); and Monoamine oxidase, MO (E.C. 1.4.3.4)] in the liver and kidney. MATERIALS AND METHODS Male Wistar albino rats were administered 1, 2 and 4 mg Cd(2+)/kg body weight for one and three months. The activities of the oxidative enzymes were subsequently analyzed in the liver and kidney after both periods of exposure. RESULTS There was a dose dependent increase in liver and kidney Cd concentration in the test rats as compared to control after both periods of treatment with the liver retaining higher concentration of Cd than the kidney for each of the exposure dose. The oxidative enzymes were decreased in a dose dependent manner in the liver and kidney after both periods of treatment. The percentage inhibition of these enzymes was less in the liver of rats treated with Cd for three months relative to the one month treated rats for each of the exposure dose. Conversely, the inhibition of the activities of these enzymes in the kidney of rats in all the treatment groups was more pronounced after three months relative to the trend in the one month treated rats. However, the activities of the oxidative enzymes were higher in the liver as compared to the kidney in all the treatment groups after both durations of Cd treatment. CONCLUSION Based on the results obtained, it can be concluded that the inhibition of the oxidative enzymes by Cd may disturb metabolism of bioactive endogenous substances, exogenous components of food and some xenobiotics.